Detailed information of TA system TA176599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 28823..29321
• Replicon: plasmid pZ869-2
• Accession: NZ_CP062784
• Organism: Escherichia coli O157:H7 strain Z869

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A764VNK7
• Locus tag: ILQ35_RS29115
• Protein ID: WP_000868275.1
• Coordinates: 29055..29321 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: A0A3H7VMJ5
• Locus tag: ILQ35_RS29110
• Protein ID: WP_001450803.1
• Coordinates: 28823..29065 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ35_RS29070	24026..24364	-	339	WP_000753495.1	hypothetical protein	-
ILQ35_RS29075	24710..25117	+	408	WP_001190619.1	hypothetical protein	-
ILQ35_RS29080	25127..25888	+	762	WP_001097937.1	conjugal transfer protein TraL	-
ILQ35_RS29085	25885..26589	+	705	WP_001279865.1	hypothetical protein	-
ILQ35_RS29090	26618..27055	-	438	WP_000466317.1	type II toxin-antitoxin system HicB family antitoxin	-
ILQ35_RS29095	27080..27262	-	183	WP_000833471.1	type II toxin-antitoxin system HicA family toxin	-
ILQ35_RS29100	27694..27972	-	279	WP_206391611.1	cobalamin biosynthesis protein CbiX	-
ILQ35_RS29105	28030..28383	-	354	WP_206391613.1	hypothetical protein	-
ILQ35_RS29110	28823..29065	-	243	WP_001450803.1	hypothetical protein	Antitoxin
ILQ35_RS29115	29055..29321	-	267	WP_000868275.1	hypothetical protein	Toxin
ILQ35_RS29120	29372..29596	-	225	WP_096991711.1	hypothetical protein	-
ILQ35_RS29125	29692..29916	-	225	WP_000866040.1	hypothetical protein	-
ILQ35_RS29130	29958..30281	-	324	WP_096991712.1	hypothetical protein	-
ILQ35_RS29135	31961..32965	+	1005	WP_001208090.1	replication initiation protein	-
ILQ35_RS29140	33449..33787	-	339	WP_000612441.1	helix-turn-helix domain-containing protein	-
ILQ35_RS29145	33986..34273	+	288	WP_000332251.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..39226	39226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10199.63 Da
• Isoelectric Point: 9.5657

>T176599 WP_000868275.1 NZ_CP062784:c29321-29055 [Escherichia coli O157:H7]
MKVRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Nucleotide

• Length: 267 bp

>T176599 NZ_CP082840:356784-357131 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9288.69 Da
• Isoelectric Point: 9.7120

>AT176599 WP_001450803.1 NZ_CP062784:c29065-28823 [Escherichia coli O157:H7]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Nucleotide

• Length: 243 bp

>AT176599 NZ_CP082840:357131-357430 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A764VNK7

Antitoxin

• AlphaFold DB: A0A3H7VMJ5