Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 26618..27262 Replicon plasmid pZ869-2
Accession NZ_CP062784
Organism Escherichia coli O157:H7 strain Z869

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag ILQ35_RS29095 Protein ID WP_000833471.1
Coordinates 27080..27262 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag ILQ35_RS29090 Protein ID WP_000466317.1
Coordinates 26618..27055 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ35_RS29070 24026..24364 - 339 WP_000753495.1 hypothetical protein -
ILQ35_RS29075 24710..25117 + 408 WP_001190619.1 hypothetical protein -
ILQ35_RS29080 25127..25888 + 762 WP_001097937.1 conjugal transfer protein TraL -
ILQ35_RS29085 25885..26589 + 705 WP_001279865.1 hypothetical protein -
ILQ35_RS29090 26618..27055 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ILQ35_RS29095 27080..27262 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
ILQ35_RS29100 27694..27972 - 279 WP_206391611.1 cobalamin biosynthesis protein CbiX -
ILQ35_RS29105 28030..28383 - 354 WP_206391613.1 hypothetical protein -
ILQ35_RS29110 28823..29065 - 243 WP_001450803.1 hypothetical protein -
ILQ35_RS29115 29055..29321 - 267 WP_000868275.1 hypothetical protein -
ILQ35_RS29120 29372..29596 - 225 WP_096991711.1 hypothetical protein -
ILQ35_RS29125 29692..29916 - 225 WP_000866040.1 hypothetical protein -
ILQ35_RS29130 29958..30281 - 324 WP_096991712.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..39226 39226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T176598 WP_000833471.1 NZ_CP062784:c27262-27080 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T176598 NZ_CP082840:330813-331181 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT176598 WP_000466317.1 NZ_CP062784:c27055-26618 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT176598 NZ_CP082840:330595-330816 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References