Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51302..51827 Replicon plasmid pZ869-1
Accession NZ_CP062783
Organism Escherichia coli O157:H7 strain Z869

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ35_RS28730 Protein ID WP_001159868.1
Coordinates 51522..51827 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ35_RS28725 Protein ID WP_000813634.1
Coordinates 51302..51520 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ35_RS28695 46585..46815 + 231 WP_010891288.1 hypothetical protein -
ILQ35_RS28700 46936..47676 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ35_RS28705 47961..48938 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ35_RS28710 49346..49546 - 201 WP_000708307.1 hypothetical protein -
ILQ35_RS28715 49543..50163 - 621 WP_001248529.1 hypothetical protein -
ILQ35_RS28720 50160..50843 - 684 WP_010891291.1 DNA-binding protein -
ILQ35_RS28725 51302..51520 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ35_RS28730 51522..51827 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ35_RS28735 51828..52634 + 807 WP_000016989.1 site-specific integrase -
ILQ35_RS28745 54532..54870 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
ILQ35_RS28750 55458..56624 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92745 92745


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176597 WP_001159868.1 NZ_CP062783:51522-51827 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176597 NZ_CP082839:c4144337-4143924 [Sphingomonas nostoxanthinifaciens]
ATGGCCGCTAGCTTTCTCGACAGTAACGTCCTGATCTATCTCGCTTCGGGGAATCCCGCGAAAGCCGACCGGGTCGAGGC
GCTGCTGGCGGCAGGTGCCTTGATCAGCGTCCAGGTCTTGAACGAGACGGCCAATGTCCTGCGGCGCAAGGCCGGATTTT
CATGGCCGGAAACGCACGCTTTCCTCGCGGCCATTCGCCCGCTTGTGGATGTCGCGCCGCTGACGATCGCGACCCATGAC
GCGGGCTTGCGCTTGGCCGAGCTTTATCGGCTGTCGCTCTACGACGCGATGATCGTCGCCGCCGCCCGGCTCGCGGGTTG
CGACATGCTGTGGTCGGAAGACATGCACGATGGGCTGGTGATCGATGACGCTTTGCGCATCCGCAACCCATTTGCGGAAA
AACCCGACACTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176597 WP_000813634.1 NZ_CP062783:51302-51520 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176597 NZ_CP082839:c4144557-4144327 [Sphingomonas nostoxanthinifaciens]
ATGCAGGTCGCCAAATGGGGTAACAGCCTGGCCGTTCGATTGCCCGCCTCGGTGGTGGAGGCGCTCGGCCTTCACGAGGG
CGATGATATCGAACTGCACGTCGCCGACACGCACAGCATGGGCGTGGCGCGCAAACCCGGCACCGACGAGCTGCTGGCGC
GGTTGCGCGCCTATCGCGGTCGCCTCCCCGCGGACTTCAAGTTCGACCGGGACGAGGCAAATGGCCGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References