Detailed information of TA system TA176586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3713146..3713624
• Replicon: chromosome
• Accession: NZ_CP062782
• Organism: Escherichia coli O157:H7 strain Z869

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: ILQ35_RS19350
• Protein ID: WP_001303876.1
• Coordinates: 3713337..3713624 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: ILQ35_RS19345
• Protein ID: WP_000536233.1
• Coordinates: 3713146..3713337 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ35_RS19310	3708550..3709320	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ35_RS19315	3709342..3710088	-	747	WP_000788751.1	ATP-binding protein	-
ILQ35_RS19320	3710095..3711186	-	1092	WP_001205823.1	hypothetical protein	-
ILQ35_RS19325	3711265..3711720	+	456	WP_000273724.1	hypothetical protein	-
ILQ35_RS19330	3711927..3712352	-	426	WP_000693855.1	Rha family transcriptional regulator	-
ILQ35_RS19335	3712336..3712608	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
ILQ35_RS19340	3712717..3713118	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
ILQ35_RS19345	3713146..3713337	+	192	WP_000536233.1	hypothetical protein	Antitoxin
ILQ35_RS19350	3713337..3713624	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ILQ35_RS19355	3713902..3714057	+	156	WP_000379575.1	DUF1391 family protein	-
ILQ35_RS19360	3714199..3714588	+	390	WP_000394511.1	hypothetical protein	-
ILQ35_RS19365	3714775..3714960	-	186	WP_001133046.1	hypothetical protein	-
ILQ35_RS19370	3715534..3715722	+	189	WP_000413705.1	cell division inhibition protein DicB	-
ILQ35_RS19375	3715719..3715910	+	192	WP_001098307.1	DUF1482 family protein	-
ILQ35_RS19380	3716004..3718475	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T176586 WP_001303876.1 NZ_CP062782:3713337-3713624 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T176586 NZ_CP082835:4040758-4041222 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT176586 WP_000536233.1 NZ_CP062782:3713146-3713337 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT176586 NZ_CP082835:4040423-4040758 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA