Detailed information of TA system TA176561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 53747..54272
• Replicon: plasmid pZ1769-1
• Accession: NZ_CP062781
• Organism: Escherichia coli O157:H7 strain Z1769

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: ILQ49_RS27580
• Protein ID: WP_001159868.1
• Coordinates: 53967..54272 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: ILQ49_RS27575
• Protein ID: WP_000813634.1
• Coordinates: 53747..53965 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ49_RS27545	49030..49260	+	231	WP_010891288.1	hypothetical protein	-
ILQ49_RS27550	49381..50121	+	741	WP_001066920.1	tyrosine-type recombinase/integrase	-
ILQ49_RS27555	50406..51383	-	978	WP_000361615.1	RepB family plasmid replication initiator protein	-
ILQ49_RS27560	51791..51991	-	201	WP_000708307.1	hypothetical protein	-
ILQ49_RS27565	51988..52608	-	621	WP_001248529.1	hypothetical protein	-
ILQ49_RS27570	52605..53288	-	684	WP_010891291.1	DNA-binding protein	-
ILQ49_RS27575	53747..53965	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
ILQ49_RS27580	53967..54272	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
ILQ49_RS27585	54273..55079	+	807	WP_000016989.1	site-specific integrase	-
ILQ49_RS27595	56977..57315	+	339	WP_071525396.1	RepB family plasmid replication initiator protein	-
ILQ49_RS27600	57903..59069	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / stcE / exeE / exeG / hlyC / hlyA / hlyA / hlyB / hlyD / hlyD / toxB	1..95224	95224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T176561 WP_001159868.1 NZ_CP062781:53967-54272 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T176561 NZ_CP082833:2413362-2413673 [Citrobacter rodentium NBRC 105723 = DSM 16636]
GTGCACATCATCTCCAAAGAGCCATTTGAGCAAGCGGCGCGGTTATTTCCAAACGACGCCATGGCCATTAGGGCGCTGTA
TCGCCTGGTAAGCGAAACAGACTTCAAATCGCCCGATGAGTTGCGCACCGCCGTGCCGAGTCTCGACAATTTTAAGTACC
GGAACAAATGGTGGGTTTTAGATGTGGGTGGTCATAATTTGCGAGTTATCGCCTACATCAACTTTGTGAATAAACGCTTC
TACGTGAAGCACATCGCCACCCACGCTGAATACGATAAGCTGACTCGCTACTATCGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT176561 WP_000813634.1 NZ_CP062781:53747-53965 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT176561 NZ_CP082833:2413670-2414089 [Citrobacter rodentium NBRC 105723 = DSM 16636]
ATGATCGCCGACACCACTAAAGCTATCGAAGCAACGAAGCAACTGGTAGCTGCCGTCCCTCTGCTGGGGGGCAGCACCTC
AGAGAAAGACTACCGCGACGCGCTGGAGCTGGTGGAGTACCTGATCGACCACGACGACGAGAACCCGCTGATCGACTTCC
TGGCAGCCAAGATTGCGGATTACGAGGACAACAGCGAACAGTTTGCGGAGTTCAACAAACGCATTGCAGAGATTCCGGTT
GGCGTCGCGCTACTGCGCACCCTGATTGACCAGCATCAGCTCTCCTACTCCGACCTGAAGAATGAGATTGGATCCAAATC
GCTGGTGAGCCAGATCCTTTCCGGCCAGCGCTCCCTGACGATCTCACACATCAAGGCGCTTTCGGAGCGGTTCGGCATCA
AGCCGCAGTGGTTTCTCTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2