Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5053827..5054324 Replicon chromosome
Accession NZ_CP062780
Organism Escherichia coli O157:H7 strain Z1769

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ILQ49_RS25180 Protein ID WP_001173343.1
Coordinates 5053827..5054000 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ILQ49_RS25185 Protein ID WP_000398619.1
Coordinates 5054028..5054324 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ49_RS25170 5050497..5051954 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
ILQ49_RS25175 5052191..5053708 + 1518 WP_001295074.1 lysine--tRNA ligase -
ILQ49_RS25180 5053827..5054000 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ILQ49_RS25185 5054028..5054324 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ILQ49_RS25190 5054551..5054823 - 273 WP_000405647.1 N-acetyltransferase -
ILQ49_RS25195 5054835..5055065 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ILQ49_RS25200 5055246..5056877 + 1632 WP_001216477.1 sensor histidine kinase -
ILQ49_RS25205 5056874..5057593 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176559 WP_001173343.1 NZ_CP062780:c5054000-5053827 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176559 NZ_CP082833:2117924-2118247 [Citrobacter rodentium NBRC 105723 = DSM 16636]
ATGTTCACCGTTATATTGCATGTCGACGTCGCCAAAGAATTAGCCGAGCTGCCGCCCATTGTTCAGGCTAAAATGATCCG
CCTGATCGATAAGTTAAAAGCGAACCCCACAGTGCTTCGGGAACCGGACAGTAAGCCCCTCGGCGCGGGACTGTTTGAAA
TACGCACTATGGGGATCGGGATCGCCCGGGGCCTGTATGTTTATCAGAAAGGTAAAAGGATCTACCTGCTGCGGGTCTTT
ATTAAAAAAACGCAGAAAACGCCGGAACACGAAATGCTGTTAGCCTTCACAAGGCTGGAGGAGATGCTCAATGAAGAAAA
CTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176559 WP_000398619.1 NZ_CP062780:c5054324-5054028 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176559 NZ_CP082833:2118234-2118521 [Citrobacter rodentium NBRC 105723 = DSM 16636]
ATGAAGAAAACTAAAAGTGTCGCCTGGGACGAGGTCAGAACAGACCTTCTGTCCAGTCCTGAGGTACAGGCTGCAATGGA
AGCAGAAGAACGTAAAGCGCGTCTGCAGGCTATGCTCGCAGAATGGCGCAATCATGCTGGCTTAACCCGCGCACAGGTTG
CAGAGAGAATGGGCGTCACGCCACCGACGGTATCCAGAATGGAGGCCAATATTGTCAGGGCCAGCCTGGAAACCATCGCC
CGCTATGCCAGAGCCTGTGGAATCAAACATCCACAAATCCTTTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References