Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4211168..4211786 Replicon chromosome
Accession NZ_CP062780
Organism Escherichia coli O157:H7 strain Z1769

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ILQ49_RS21310 Protein ID WP_001291435.1
Coordinates 4211568..4211786 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ILQ49_RS21305 Protein ID WP_000344800.1
Coordinates 4211168..4211542 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ49_RS21295 4206257..4207450 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ILQ49_RS21300 4207473..4210622 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
ILQ49_RS21305 4211168..4211542 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ILQ49_RS21310 4211568..4211786 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ILQ49_RS21315 4211958..4212509 + 552 WP_000102553.1 maltose O-acetyltransferase -
ILQ49_RS21320 4212625..4213095 + 471 WP_000136192.1 YlaC family protein -
ILQ49_RS21325 4213259..4214809 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ILQ49_RS21330 4214851..4215204 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ILQ49_RS21340 4215583..4215894 + 312 WP_000409911.1 MGMT family protein -
ILQ49_RS21345 4215927..4216499 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T176552 WP_001291435.1 NZ_CP062780:4211568-4211786 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T176552 NZ_CP082833:379268-379645 [Citrobacter rodentium NBRC 105723 = DSM 16636]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAACAGCATATTG
AGGCGGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCTCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT176552 WP_000344800.1 NZ_CP062780:4211168-4211542 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT176552 NZ_CP082833:378811-379179 [Citrobacter rodentium NBRC 105723 = DSM 16636]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References