Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48844..49369 Replicon plasmid pZ1836-1
Accession NZ_CP062779
Organism Escherichia coli O157:H7 strain Z1836

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ64_RS27795 Protein ID WP_001159868.1
Coordinates 49064..49369 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ64_RS27790 Protein ID WP_000813634.1
Coordinates 48844..49062 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ64_RS27760 44127..44357 + 231 WP_010891288.1 hypothetical protein -
ILQ64_RS27765 44478..45218 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ64_RS27770 45503..46480 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ64_RS27775 46888..47088 - 201 WP_000708307.1 hypothetical protein -
ILQ64_RS27780 47085..47705 - 621 WP_001248529.1 hypothetical protein -
ILQ64_RS27785 47702..48385 - 684 WP_010891291.1 DNA-binding protein -
ILQ64_RS27790 48844..49062 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ64_RS27795 49064..49369 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ64_RS27800 49370..50176 + 807 WP_024165790.1 site-specific integrase -
ILQ64_RS27810 52188..52943 + 756 WP_021499966.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / toxB 1..90783 90783


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176525 WP_001159868.1 NZ_CP062779:49064-49369 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176525 NZ_CP082829:c31314-31162 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176525 WP_000813634.1 NZ_CP062779:48844-49062 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176525 NZ_CP082829:31366-31426 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References