Detailed information of TA system TA176515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3717519..3718224
• Replicon: chromosome
• Accession: NZ_CP062778
• Organism: Escherichia coli O157:H7 strain Z1836

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: ILQ64_RS18995
• Protein ID: WP_000539519.1
• Coordinates: 3717519..3717905 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ILQ64_RS19000
• Protein ID: WP_001280945.1
• Coordinates: 3717895..3718224 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ64_RS18975	3713523..3714149	+	627	WP_001295292.1	glutathione S-transferase GstB	-
ILQ64_RS18980	3714146..3715261	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
ILQ64_RS18985	3715372..3715755	-	384	WP_000497139.1	biofilm formation regulator BssR	-
ILQ64_RS18990	3715968..3717293	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
ILQ64_RS18995	3717519..3717905	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ILQ64_RS19000	3717895..3718224	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
ILQ64_RS19005	3718294..3719622	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
ILQ64_RS19010	3719630..3721978	-	2349	WP_000950320.1	EAL domain-containing protein	-
ILQ64_RS19015	3722156..3723067	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T176515 WP_000539519.1 NZ_CP062778:3717519-3717905 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T176515 NZ_CP082827:2808651-2808758 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT176515 WP_001280945.1 NZ_CP062778:3717895-3718224 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT176515 NZ_CP082827:c2808598-2808537 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT