176488

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 25158..25656 Replicon plasmid pZ563-2
Accession NZ_CP062776
Organism Escherichia coli O157:H7 strain Z563

Toxin (Protein)


Gene name TsxA Uniprot ID A0A764VNK7
Locus tag ILQ31_RS27850 Protein ID WP_000868275.1
Coordinates 25390..25656 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A3H7VMJ5
Locus tag ILQ31_RS27845 Protein ID WP_001450803.1
Coordinates 25158..25400 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ31_RS27805 20386..20721 - 336 WP_000963832.1 hypothetical protein -
ILQ31_RS27810 21067..21474 + 408 WP_064261963.1 hypothetical protein -
ILQ31_RS27815 21484..22245 + 762 WP_001097938.1 conjugal transfer protein TraL -
ILQ31_RS27820 22242..22946 + 705 WP_001279864.1 hypothetical protein -
ILQ31_RS27825 22975..23412 - 438 WP_052989980.1 type II toxin-antitoxin system HicB family antitoxin -
ILQ31_RS27830 23437..23619 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin -
ILQ31_RS27835 24053..24331 - 279 WP_000545934.1 hypothetical protein -
ILQ31_RS27840 24389..24718 - 330 WP_000449469.1 hypothetical protein -
ILQ31_RS27845 25158..25400 - 243 WP_001450803.1 hypothetical protein Antitoxin
ILQ31_RS27850 25390..25656 - 267 WP_000868275.1 hypothetical protein Toxin
ILQ31_RS27855 25707..25931 - 225 WP_000814384.1 hypothetical protein -
ILQ31_RS27860 26029..26238 - 210 WP_000866037.1 hypothetical protein -
ILQ31_RS27865 26279..26602 - 324 WP_000427402.1 hypothetical protein -
ILQ31_RS27870 28277..29281 + 1005 WP_001208090.1 replication initiation protein -
ILQ31_RS27875 29699..30037 - 339 WP_000612441.1 helix-turn-helix domain-containing protein -
ILQ31_RS27880 30236..30523 + 288 WP_000332248.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35476 35476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10199.63 Da        Isoelectric Point: 9.5657

>T176488 WP_000868275.1 NZ_CP062776:c25656-25390 [Escherichia coli O157:H7]
MKVRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Download         Length: 267 bp

>T176488 NZ_CP082824:2279328-2279430 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9288.69 Da        Isoelectric Point: 9.7120

>AT176488 WP_001450803.1 NZ_CP062776:c25400-25158 [Escherichia coli O157:H7]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Download         Length: 243 bp

>AT176488 NZ_CP082824:c2279468-2279200 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A764VNK7

Antitoxin

Source ID Structure
AlphaFold DB A0A3H7VMJ5

References