Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 22975..23619 Replicon plasmid pZ563-2
Accession NZ_CP062776
Organism Escherichia coli O157:H7 strain Z563

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag ILQ31_RS27830 Protein ID WP_000833471.1
Coordinates 23437..23619 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2X7JR29
Locus tag ILQ31_RS27825 Protein ID WP_052989980.1
Coordinates 22975..23412 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ31_RS27805 20386..20721 - 336 WP_000963832.1 hypothetical protein -
ILQ31_RS27810 21067..21474 + 408 WP_064261963.1 hypothetical protein -
ILQ31_RS27815 21484..22245 + 762 WP_001097938.1 conjugal transfer protein TraL -
ILQ31_RS27820 22242..22946 + 705 WP_001279864.1 hypothetical protein -
ILQ31_RS27825 22975..23412 - 438 WP_052989980.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ILQ31_RS27830 23437..23619 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
ILQ31_RS27835 24053..24331 - 279 WP_000545934.1 hypothetical protein -
ILQ31_RS27840 24389..24718 - 330 WP_000449469.1 hypothetical protein -
ILQ31_RS27845 25158..25400 - 243 WP_001450803.1 hypothetical protein -
ILQ31_RS27850 25390..25656 - 267 WP_000868275.1 hypothetical protein -
ILQ31_RS27855 25707..25931 - 225 WP_000814384.1 hypothetical protein -
ILQ31_RS27860 26029..26238 - 210 WP_000866037.1 hypothetical protein -
ILQ31_RS27865 26279..26602 - 324 WP_000427402.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35476 35476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T176487 WP_000833471.1 NZ_CP062776:c23619-23437 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T176487 NZ_CP082824:c1983502-1983128 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16199.09 Da        Isoelectric Point: 4.3135

>AT176487 WP_052989980.1 NZ_CP062776:c23412-22975 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVKESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT176487 NZ_CP082824:c1983959-1983591 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A2X7JR29

References