Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5042128..5042625 Replicon chromosome
Accession NZ_CP062774
Organism Escherichia coli O157:H7 strain Z563

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ILQ31_RS25095 Protein ID WP_001173343.1
Coordinates 5042128..5042301 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ILQ31_RS25100 Protein ID WP_000398619.1
Coordinates 5042329..5042625 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ31_RS25085 5038798..5040255 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
ILQ31_RS25090 5040492..5042009 + 1518 WP_001295074.1 lysine--tRNA ligase -
ILQ31_RS25095 5042128..5042301 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ILQ31_RS25100 5042329..5042625 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ILQ31_RS25105 5042852..5043124 - 273 WP_000405647.1 N-acetyltransferase -
ILQ31_RS25110 5043136..5043366 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ILQ31_RS25115 5043547..5045178 + 1632 WP_001216477.1 sensor histidine kinase -
ILQ31_RS25120 5045175..5045894 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176484 WP_001173343.1 NZ_CP062774:c5042301-5042128 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176484 NZ_CP082824:1100816-1101151 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176484 WP_000398619.1 NZ_CP062774:c5042625-5042329 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176484 NZ_CP082824:1100568-1100816 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References