Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4694772..4695322 Replicon chromosome
Accession NZ_CP062774
Organism Escherichia coli O157:H7 strain Z563

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag ILQ31_RS23535 Protein ID WP_001160975.1
Coordinates 4694772..4695086 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ILQ31_RS23540 Protein ID WP_000125566.1
Coordinates 4695089..4695322 (-) Length 78 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ31_RS23510 4690447..4691733 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
ILQ31_RS23515 4691733..4692722 + 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ILQ31_RS23520 4692719..4693540 + 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ILQ31_RS23525 4693543..4693920 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ILQ31_RS23530 4693927..4694775 + 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ILQ31_RS23535 4694772..4695086 - 315 WP_001160975.1 CcdB family protein Toxin
ILQ31_RS23540 4695089..4695322 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ILQ31_RS23545 4695408..4695887 - 480 WP_000624372.1 type 3 dihydrofolate reductase -
ILQ31_RS23550 4696079..4697941 - 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
ILQ31_RS23555 4697934..4698464 - 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ILQ31_RS23560 4698572..4699903 - 1332 WP_001183177.1 MFS transporter -
ILQ31_RS23565 4699961..4700248 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-103)

Antitoxin

(8-77)


Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T176480 WP_001160975.1 NZ_CP062774:c4695086-4694772 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T176480 NZ_CP062774:c4695086-4694772 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT176480 WP_000125566.1 NZ_CP062774:c4695322-4695089 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT176480 NZ_CP062774:c4695322-4695089 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References