Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5093093..5093590 Replicon chromosome
Accession NZ_CP062771
Organism Escherichia coli O157:H7 strain Z570

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ILQ32_RS25445 Protein ID WP_001173343.1
Coordinates 5093093..5093266 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ILQ32_RS25450 Protein ID WP_000398619.1
Coordinates 5093294..5093590 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ32_RS25435 5089763..5091220 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
ILQ32_RS25440 5091457..5092974 + 1518 WP_001295074.1 lysine--tRNA ligase -
ILQ32_RS25445 5093093..5093266 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ILQ32_RS25450 5093294..5093590 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ILQ32_RS25455 5093817..5094089 - 273 WP_000405647.1 N-acetyltransferase -
ILQ32_RS25460 5094101..5094331 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ILQ32_RS25465 5094512..5096143 + 1632 WP_001216477.1 sensor histidine kinase -
ILQ32_RS25470 5096140..5096859 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176446 WP_001173343.1 NZ_CP062771:c5093266-5093093 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176446 NZ_CP082820:c1592904-1592650 [Streptococcus pneumoniae]
ATGCTGCTCAAGTTTACAGAAGATGCCTGGGCAGATTATTGCTACTGGCAAAATCAGGATAAGAAAACGTTAAAAAGAAT
CAATAAACTAATCAAGGATATTCAACGTGATCCCTTTACAGGAATAGGTAAACCAGAACCACTCAAGTATGACTACCAAG
GAGCTTGCTCACGTCGTATTGATGCAGAAAATCGCTTGATTTATATGATGGATGGAGATAGCGTGGCTTTCTTGTCCTTT
AAAGATCATTACTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176446 WP_000398619.1 NZ_CP062771:c5093590-5093294 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176446 NZ_CP082820:c1593162-1592908 [Streptococcus pneumoniae]
ATGGAAGCAGTCCTTTACTCAACATTCCGAAATCATTTAAAGGACTACATGAAGAAGGTAAATGATGAATTTGAGCCTTT
GACGGTGGTCAATAAAAATCCAGACGAGGACATTGTAGTCCTTTCAAAGAGTGAGTGGGATAGTATCCAAGAAACCCTGA
GAATTGCTCAAAATAAGGAACTTTCTGATAAGGTTTTGCGAGGAATGGCTCAAGTTCGTGCTGGAAGTACTCAGGTCCAT
GTTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References