Detailed information of TA system TA176445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4996102..4996697
• Replicon: chromosome
• Accession: NZ_CP062771
• Organism: Escherichia coli O157:H7 strain Z570

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: ILQ32_RS24945
• Protein ID: WP_000239579.1
• Coordinates: 4996102..4996452 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: ILQ32_RS24950
• Protein ID: WP_001223210.1
• Coordinates: 4996446..4996697 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ32_RS24925	4991548..4992570	-	1023	WP_001301928.1	ABC transporter permease	-
ILQ32_RS24930	4992584..4994086	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
ILQ32_RS24935	4994226..4995182	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ILQ32_RS24940	4995492..4996022	+	531	WP_000055075.1	inorganic diphosphatase	-
ILQ32_RS24945	4996102..4996452	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
ILQ32_RS24950	4996446..4996697	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
ILQ32_RS24955	4996909..4997250	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
ILQ32_RS24960	4997253..5001032	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T176445 WP_000239579.1 NZ_CP062771:c4996452-4996102 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T176445 NZ_CP082820:c1131477-1130701 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAATCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAAAATGGCAAAGATAGCGTTGAATACACCAAAGATTTTGCAGGGAAAATGGTAGAGTCTTTAGTAACCGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACTATTGATGTTCCAAAGAAAACAGCACAACTATTAAAAAATA
AGGGGTATGAGGTACAATTAGCCTTAATTGCAACCAAGCCCGAATTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTGTATGCTATCAATCCAAATCCAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATTTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGAAGTGCTACAAGAATACCTCTTCGGAAAATGGAGCAAGGTAGAGGAAGAGATGTTG
AAGGTGGGAGAAGAACGGATGAGGGATATGAGTAAAAGTAATGGAAAAGATGCATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT176445 WP_001223210.1 NZ_CP062771:c4996697-4996446 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT176445 NZ_CP082820:c1131953-1131477 [Streptococcus pneumoniae]
ATGATTGGAAAAAACATAAAATCCTTACGTAAAACACATGACTTAACACAACACGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACGGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCAGACAAATATCTATTTAGTAGAAAATTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8