Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4250444..4251062 Replicon chromosome
Accession NZ_CP062771
Organism Escherichia coli O157:H7 strain Z570

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ILQ32_RS21575 Protein ID WP_001291435.1
Coordinates 4250844..4251062 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ILQ32_RS21570 Protein ID WP_000344800.1
Coordinates 4250444..4250818 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ32_RS21560 4245533..4246726 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ILQ32_RS21565 4246749..4249898 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
ILQ32_RS21570 4250444..4250818 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ILQ32_RS21575 4250844..4251062 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ILQ32_RS21580 4251234..4251785 + 552 WP_000102553.1 maltose O-acetyltransferase -
ILQ32_RS21585 4251901..4252371 + 471 WP_000136192.1 YlaC family protein -
ILQ32_RS21590 4252535..4254085 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ILQ32_RS21595 4254127..4254480 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ILQ32_RS21605 4254859..4255170 + 312 WP_000409911.1 MGMT family protein -
ILQ32_RS21610 4255203..4255775 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T176439 WP_001291435.1 NZ_CP062771:4250844-4251062 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T176439 NZ_CP082815:c2211399-2211037 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT176439 WP_000344800.1 NZ_CP062771:4250444-4250818 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT176439 NZ_CP082815:c2211566-2211396 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References