Detailed information of TA system TA176433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2848358..2848996
• Replicon: chromosome
• Accession: NZ_CP062771
• Organism: Escherichia coli O157:H7 strain Z570

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ILQ32_RS14480
• Protein ID: WP_000813794.1
• Coordinates: 2848820..2848996 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ILQ32_RS14475
• Protein ID: WP_001270286.1
• Coordinates: 2848358..2848774 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ32_RS14455	2843510..2844451	-	942	WP_001251315.1	ABC transporter permease	-
ILQ32_RS14460	2844452..2845465	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
ILQ32_RS14465	2845483..2846628	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
ILQ32_RS14470	2846873..2848279	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
ILQ32_RS14475	2848358..2848774	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ILQ32_RS14480	2848820..2848996	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ILQ32_RS14485	2849218..2849448	+	231	WP_000494244.1	YncJ family protein	-
ILQ32_RS14490	2849540..2851501	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ILQ32_RS14495	2851574..2852110	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
ILQ32_RS14500	2852163..2853374	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T176433 WP_000813794.1 NZ_CP062771:c2848996-2848820 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T176433 NZ_CP082815:c848386-848198 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT176433 WP_001270286.1 NZ_CP062771:c2848774-2848358 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT176433 NZ_CP082815:c848675-848415 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• PDB: 6HPB