Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 365365..365996 Replicon chromosome
Accession NZ_CP062771
Organism Escherichia coli O157:H7 strain Z570

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag ILQ32_RS01665 Protein ID WP_001259386.1
Coordinates 365365..365640 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ILQ32_RS01670 Protein ID WP_000593555.1
Coordinates 365637..365996 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ32_RS01650 360369..361436 + 1068 WP_000361458.1 HlyD family secretion protein -
ILQ32_RS01655 361433..364168 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
ILQ32_RS01660 364168..365292 + 1125 WP_001301659.1 ABC transporter permease -
ILQ32_RS01665 365365..365640 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
ILQ32_RS01670 365637..365996 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ILQ32_RS01675 366046..366906 - 861 WP_001131788.1 class II aldolase -
ILQ32_RS01680 366938..367207 - 270 WP_000933060.1 HPr family phosphocarrier protein -
ILQ32_RS01685 367197..368705 - 1509 WP_001301773.1 carbohydrate kinase -
ILQ32_RS01690 368698..370056 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ILQ32_RS01695 370133..370414 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ILQ32_RS01700 370411..370884 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 356509..365996 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T176414 WP_001259386.1 NZ_CP062771:365365-365640 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T176414 NZ_CP082806:70241-70684 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT176414 WP_000593555.1 NZ_CP062771:365637-365996 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT176414 NZ_CP082806:70014-70244 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References