Detailed information of TA system TA176375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4753164..4753714
• Replicon: chromosome
• Accession: NZ_CP062766
• Organism: Escherichia coli O157:H7 strain Z866

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: ILQ34_RS23885
• Protein ID: WP_001160975.1
• Coordinates: 4753164..4753478 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: ILQ34_RS23890
• Protein ID: WP_000125566.1
• Coordinates: 4753481..4753714 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ34_RS23860	4748839..4750125	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
ILQ34_RS23865	4750125..4751114	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
ILQ34_RS23870	4751111..4751932	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
ILQ34_RS23875	4751935..4752312	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
ILQ34_RS23880	4752319..4753167	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
ILQ34_RS23885	4753164..4753478	-	315	WP_001160975.1	CcdB family protein	Toxin
ILQ34_RS23890	4753481..4753714	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
ILQ34_RS23895	4753800..4754279	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
ILQ34_RS23900	4754471..4756333	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
ILQ34_RS23905	4756326..4756856	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
ILQ34_RS23910	4756964..4758295	-	1332	WP_001183177.1	MFS transporter	-
ILQ34_RS23915	4758353..4758640	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T176375 WP_001160975.1 NZ_CP062766:c4753478-4753164 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T176375 NZ_CP082796:315916-316284 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT176375 WP_000125566.1 NZ_CP062766:c4753714-4753481 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT176375 NZ_CP082796:315698-315919 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9