Detailed information of TA system TA176371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3800511..3801216
• Replicon: chromosome
• Accession: NZ_CP062766
• Organism: Escherichia coli O157:H7 strain Z866

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: ILQ34_RS19485
• Protein ID: WP_000539519.1
• Coordinates: 3800511..3800897 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ILQ34_RS19490
• Protein ID: WP_001280945.1
• Coordinates: 3800887..3801216 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ34_RS19465	3796515..3797141	+	627	WP_001295292.1	glutathione S-transferase GstB	-
ILQ34_RS19470	3797138..3798253	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
ILQ34_RS19475	3798364..3798747	-	384	WP_000497137.1	biofilm formation regulator BssR	-
ILQ34_RS19480	3798960..3800285	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
ILQ34_RS19485	3800511..3800897	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ILQ34_RS19490	3800887..3801216	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
ILQ34_RS19495	3801286..3802614	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
ILQ34_RS19500	3802622..3804970	-	2349	WP_000950320.1	EAL domain-containing protein	-
ILQ34_RS19505	3805148..3806059	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T176371 WP_000539519.1 NZ_CP062766:3800511-3800897 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T176371 NZ_CP082791:5166552-5166654 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT176371 WP_001280945.1 NZ_CP062766:3800887-3801216 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT176371 NZ_CP082791:c5166656-5166516 [Klebsiella pneumoniae]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT