Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5069165..5069662 Replicon chromosome
Accession NZ_CP062763
Organism Escherichia coli O157:H7 strain Z885

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ILQ36_RS25235 Protein ID WP_001173343.1
Coordinates 5069165..5069338 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ILQ36_RS25240 Protein ID WP_000398619.1
Coordinates 5069366..5069662 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ36_RS25225 5065835..5067292 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
ILQ36_RS25230 5067529..5069046 + 1518 WP_001295074.1 lysine--tRNA ligase -
ILQ36_RS25235 5069165..5069338 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ILQ36_RS25240 5069366..5069662 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ILQ36_RS25245 5069889..5070161 - 273 WP_000405647.1 N-acetyltransferase -
ILQ36_RS25250 5070173..5070403 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ILQ36_RS25255 5070584..5072215 + 1632 WP_001216477.1 sensor histidine kinase -
ILQ36_RS25260 5072212..5072931 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176343 WP_001173343.1 NZ_CP062763:c5069338-5069165 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176343 NZ_CP082787:c1131297-1131094 [Serratia entomophila]
ATGACAAAAATCGACTATCTAATGCGTTTGCGTAAATGCACCACCATTGACACCCTCGAACGCGTTATTGAGAAGAATAA
GTATGAGCTTTCCGACGATGAGCTGGAGCTGTTTTACTCGGCCGCCGATCATCGCCTGGCGGAACTCACCATGAACAAAC
TGTATGATAAAATCCCCACGGCCGTTTGGAAGTACGTAAGATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176343 WP_000398619.1 NZ_CP062763:c5069662-5069366 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176343 NZ_CP082787:c1131717-1131349 [Serratia entomophila]
ATGGATGAGTATTCGCCTAAGCGACATGATATTGCTCAACTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGCATCGC
CACCCTGGGAGACAGCCACCACGGGTGGGTGAACGATCCAACCTCTTCCGTCAACCTCCAGCTTAATGAGTTGATTGAAC
ATATTGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGATGAAACAGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGTTGTTCAGCAGCTACGGTATCAATGATTCCGATCTTCGTCGTTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGGCATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References