Detailed information of TA system TA176342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4972174..4972769
• Replicon: chromosome
• Accession: NZ_CP062763
• Organism: Escherichia coli O157:H7 strain Z885

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: ILQ36_RS24735
• Protein ID: WP_000239579.1
• Coordinates: 4972174..4972524 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: ILQ36_RS24740
• Protein ID: WP_001223210.1
• Coordinates: 4972518..4972769 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ36_RS24715	4967620..4968642	-	1023	WP_001301928.1	ABC transporter permease	-
ILQ36_RS24720	4968656..4970158	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
ILQ36_RS24725	4970298..4971254	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ILQ36_RS24730	4971564..4972094	+	531	WP_000055075.1	inorganic diphosphatase	-
ILQ36_RS24735	4972174..4972524	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
ILQ36_RS24740	4972518..4972769	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
ILQ36_RS24745	4972981..4973322	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
ILQ36_RS24750	4973325..4977104	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T176342 WP_000239579.1 NZ_CP062763:c4972524-4972174 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T176342 NZ_CP082787:439615-439890 [Serratia entomophila]
ATGCGTATCGAATGGGATAATGCAGCGCTACGGGACAGGGAACGTATTTTTGAGTTTCTTTATATTTTCAATCCACAGGC
GGCAGAGCAGGCTGATAACGAGATCGATAAAGCGGTAAAGCGCCTGCTGGATCATCCTGAGTTAGGTAAGCCCTGGTATG
GGAAGGCACGTAAGCTGCTAATTAACAAGGCCTCCCTGTTGCTGGTGTATATCATCGTTGATGACGTTATTAAGGTTTTC
TCGATTGCCCACCAGCGTGAGAAATTTCCAAACTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT176342 WP_001223210.1 NZ_CP062763:c4972769-4972518 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT176342 NZ_CP082787:439319-439606 [Serratia entomophila]
ATGGAAAGTCGTATCCAGTTTCGCATCGAAGATGAAACCAAGCGTTTGGCGCAGAAGGCCGCTGAGGCTAAAGGAACGAC
ATTGAGTGAAGCCTGCCGCCGCTTAGCGGAGCAAATGGCTGATGAGCAACGGGCCGCGGAGGAGCACGAAAACTGGCTGA
AAGATAAGGTGGATGCAGCTTTTTCCCGTCTGCATGAAGGTAATGCTGTCTACCTTAGCCAACAAGAAGTAGAAGAAAGC
ATGGATGCCTTTAAGGCAAAAATTAGAGCGAAATACGACCGAAAATAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8