Detailed information of TA system TA176335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3770482..3771187
• Replicon: chromosome
• Accession: NZ_CP062763
• Organism: Escherichia coli O157:H7 strain Z885

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: ILQ36_RS19285
• Protein ID: WP_000539519.1
• Coordinates: 3770482..3770868 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ILQ36_RS19290
• Protein ID: WP_001280945.1
• Coordinates: 3770858..3771187 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ36_RS19265	3766486..3767112	+	627	WP_001295292.1	glutathione S-transferase GstB	-
ILQ36_RS19270	3767109..3768224	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
ILQ36_RS19275	3768335..3768718	-	384	WP_000497137.1	biofilm formation regulator BssR	-
ILQ36_RS19280	3768931..3770256	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
ILQ36_RS19285	3770482..3770868	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ILQ36_RS19290	3770858..3771187	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
ILQ36_RS19295	3771257..3772585	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
ILQ36_RS19300	3772593..3774941	-	2349	WP_000950320.1	EAL domain-containing protein	-
ILQ36_RS19305	3775119..3776030	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T176335 WP_000539519.1 NZ_CP062763:3770482-3770868 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T176335 NZ_CP082785:1837373-1837475 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT176335 WP_001280945.1 NZ_CP062763:3770858-3771187 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT176335 NZ_CP082785:c1837479-1837332 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG