Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51300..51825 Replicon plasmid pZ887-1
Accession NZ_CP062762
Organism Escherichia coli O157:H7 strain Z887

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ37_RS27675 Protein ID WP_001159868.1
Coordinates 51520..51825 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ37_RS27670 Protein ID WP_000813634.1
Coordinates 51300..51518 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ37_RS27640 46583..46813 + 231 WP_010891288.1 hypothetical protein -
ILQ37_RS27645 46934..47674 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ37_RS27650 47959..48936 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ37_RS27655 49344..49544 - 201 WP_000708307.1 hypothetical protein -
ILQ37_RS27660 49541..50161 - 621 WP_001248529.1 hypothetical protein -
ILQ37_RS27665 50158..50841 - 684 WP_010891291.1 DNA-binding protein -
ILQ37_RS27670 51300..51518 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ37_RS27675 51520..51825 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ37_RS27680 51826..52632 + 807 WP_000016989.1 site-specific integrase -
ILQ37_RS27690 54530..54868 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
ILQ37_RS27695 55456..56622 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92756 92756


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176307 WP_001159868.1 NZ_CP062762:51520-51825 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176307 NZ_CP082777:525196-525369 [Escherichia coli O25:H1]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176307 WP_000813634.1 NZ_CP062762:51300-51518 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176307 NZ_CP082777:524872-525168 [Escherichia coli O25:H1]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References