Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5073030..5073527 Replicon chromosome
Accession NZ_CP062761
Organism Escherichia coli O157:H7 strain Z887

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ILQ37_RS25290 Protein ID WP_001173343.1
Coordinates 5073030..5073203 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ILQ37_RS25295 Protein ID WP_000398619.1
Coordinates 5073231..5073527 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ37_RS25280 5069700..5071157 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
ILQ37_RS25285 5071394..5072911 + 1518 WP_001295074.1 lysine--tRNA ligase -
ILQ37_RS25290 5073030..5073203 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ILQ37_RS25295 5073231..5073527 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ILQ37_RS25300 5073754..5074026 - 273 WP_000405647.1 N-acetyltransferase -
ILQ37_RS25305 5074038..5074268 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ILQ37_RS25310 5074449..5076080 + 1632 WP_001216477.1 sensor histidine kinase -
ILQ37_RS25315 5076077..5076796 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176305 WP_001173343.1 NZ_CP062761:c5073203-5073030 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176305 NZ_CP082776:c4618152-4618045 [Escherichia coli O4:H5]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176305 WP_000398619.1 NZ_CP062761:c5073527-5073231 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176305 NZ_CP082776:4618209-4618266 [Escherichia coli O4:H5]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References