Detailed information of TA system TA176273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 431607..433935
• Replicon: chromosome
• Accession: NZ_CP062761
• Organism: Escherichia coli O157:H7 strain Z887

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: ILQ37_RS02035
• Protein ID: WP_000532146.1
• Coordinates: 431607..432581 (+)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: ILQ37_RS02040
• Protein ID: WP_001303704.1
• Coordinates: 432640..433935 (+)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ37_RS02010	427464..428459	+	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-
ILQ37_RS02015	428598..429125	+	528	WP_000108330.1	gluconokinase	-
ILQ37_RS02020	429129..430469	+	1341	WP_000210111.1	gluconate transporter	-
ILQ37_RS02025	430609..431217	+	609	WP_000410808.1	ATP-dependent DNA helicase	-
ILQ37_RS02030	431202..431603	+	402	WP_000502498.1	ATP-dependent DNA helicase	-
ILQ37_RS02035	431607..432581	+	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
ILQ37_RS02040	432640..433935	+	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
ILQ37_RS02045	433981..434574	-	594	WP_001002544.1	NAAT family transporter YhgN	-
ILQ37_RS02050	434766..435869	+	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
ILQ37_RS02055	436142..438328	+	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T176273 WP_000532146.1 NZ_CP062761:431607-432581 [Escherichia coli O157:H7]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T176273 NZ_CP082775:775813-776097 [Escherichia coli O25:H4]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT176273 WP_001303704.1 NZ_CP062761:432640-433935 [Escherichia coli O157:H7]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT176273 NZ_CP082775:775577-775810 [Escherichia coli O25:H4]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8