Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 365364..365995 Replicon chromosome
Accession NZ_CP062761
Organism Escherichia coli O157:H7 strain Z887

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag ILQ37_RS01665 Protein ID WP_001259386.1
Coordinates 365364..365639 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ILQ37_RS01670 Protein ID WP_000593555.1
Coordinates 365636..365995 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ37_RS01650 360368..361435 + 1068 WP_000361458.1 HlyD family secretion protein -
ILQ37_RS01655 361432..364167 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
ILQ37_RS01660 364167..365291 + 1125 WP_001301659.1 ABC transporter permease -
ILQ37_RS01665 365364..365639 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
ILQ37_RS01670 365636..365995 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ILQ37_RS01675 366045..366905 - 861 WP_001131788.1 class II aldolase -
ILQ37_RS01680 366937..367206 - 270 WP_000933060.1 HPr family phosphocarrier protein -
ILQ37_RS01685 367196..368704 - 1509 WP_001301773.1 carbohydrate kinase -
ILQ37_RS01690 368697..370055 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ILQ37_RS01695 370132..370413 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ILQ37_RS01700 370410..370883 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 356508..365995 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T176271 WP_001259386.1 NZ_CP062761:365364-365639 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T176271 NZ_CP082775:484909-485082 [Escherichia coli O25:H4]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT176271 WP_000593555.1 NZ_CP062761:365636-365995 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT176271 NZ_CP082775:484585-484881 [Escherichia coli O25:H4]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References