176269

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51304..51829 Replicon plasmid pZ892-1
Accession NZ_CP062759
Organism Escherichia coli O157:H7 strain Z892

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ38_RS27505 Protein ID WP_001159868.1
Coordinates 51524..51829 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ38_RS27500 Protein ID WP_000813634.1
Coordinates 51304..51522 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ38_RS27470 46587..46817 + 231 WP_010891288.1 hypothetical protein -
ILQ38_RS27475 46938..47678 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ38_RS27480 47963..48940 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ38_RS27485 49348..49548 - 201 WP_000708307.1 hypothetical protein -
ILQ38_RS27490 49545..50165 - 621 WP_001248529.1 hypothetical protein -
ILQ38_RS27495 50162..50845 - 684 WP_010891291.1 DNA-binding protein -
ILQ38_RS27500 51304..51522 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ38_RS27505 51524..51829 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ38_RS27510 51830..52636 + 807 WP_000016989.1 site-specific integrase -
ILQ38_RS27520 54534..54872 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
ILQ38_RS27525 55460..56626 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92766 92766


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176269 WP_001159868.1 NZ_CP062759:51524-51829 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176269 NZ_CP082774:c4789739-4789632 [Escherichia coli O16:H6]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCAGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176269 WP_000813634.1 NZ_CP062759:51304-51522 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176269 NZ_CP082774:4789787-4789853 [Escherichia coli O16:H6]
GGTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References