Detailed information of TA system TA176258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3581917..3582395
• Replicon: chromosome
• Accession: NZ_CP062758
• Organism: Escherichia coli O157:H7 strain Z892

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: ILQ38_RS18425
• Protein ID: WP_001303876.1
• Coordinates: 3582108..3582395 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: ILQ38_RS18420
• Protein ID: WP_000536233.1
• Coordinates: 3581917..3582108 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ38_RS18385	3577321..3578091	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ38_RS18390	3578113..3578859	-	747	WP_000788751.1	ATP-binding protein	-
ILQ38_RS18395	3578866..3579957	-	1092	WP_001205823.1	hypothetical protein	-
ILQ38_RS18400	3580036..3580491	+	456	WP_000273724.1	hypothetical protein	-
ILQ38_RS18405	3580698..3581123	-	426	WP_000693855.1	Rha family transcriptional regulator	-
ILQ38_RS18410	3581107..3581379	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
ILQ38_RS18415	3581488..3581889	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
ILQ38_RS18420	3581917..3582108	+	192	WP_000536233.1	hypothetical protein	Antitoxin
ILQ38_RS18425	3582108..3582395	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ILQ38_RS18430	3582673..3582828	+	156	WP_000379575.1	DUF1391 family protein	-
ILQ38_RS18435	3582970..3583359	+	390	WP_000394511.1	hypothetical protein	-
ILQ38_RS18440	3583546..3583731	-	186	WP_001133046.1	hypothetical protein	-
ILQ38_RS18445	3584305..3584493	+	189	WP_000413705.1	cell division inhibition protein DicB	-
ILQ38_RS18450	3584490..3584681	+	192	WP_001098307.1	DUF1482 family protein	-
ILQ38_RS18455	3584775..3587237	+	2463	WP_206396588.1	3'-5' exoribonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T176258 WP_001303876.1 NZ_CP062758:3582108-3582395 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T176258 NZ_CP082774:1202331-1202729 [Escherichia coli O16:H6]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT176258 WP_000536233.1 NZ_CP062758:3581917-3582108 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT176258 NZ_CP082774:1202035-1202328 [Escherichia coli O16:H6]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA