Detailed information of TA system TA176252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2790515..2791153
• Replicon: chromosome
• Accession: NZ_CP062758
• Organism: Escherichia coli O157:H7 strain Z892

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ILQ38_RS14050
• Protein ID: WP_000813794.1
• Coordinates: 2790515..2790691 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ILQ38_RS14055
• Protein ID: WP_001270286.1
• Coordinates: 2790737..2791153 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ38_RS14030	2786137..2787348	-	1212	WP_071525382.1	BenE family transporter YdcO	-
ILQ38_RS14035	2787401..2787937	+	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
ILQ38_RS14040	2788010..2789971	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ILQ38_RS14045	2790063..2790293	-	231	WP_000494244.1	YncJ family protein	-
ILQ38_RS14050	2790515..2790691	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ILQ38_RS14055	2790737..2791153	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ILQ38_RS14060	2791232..2792638	+	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
ILQ38_RS14065	2792883..2794028	+	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
ILQ38_RS14070	2794046..2795059	+	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
ILQ38_RS14075	2795060..2796001	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T176252 WP_000813794.1 NZ_CP062758:2790515-2790691 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T176252 NZ_CP082774:480160-480333 [Escherichia coli O16:H6]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT176252 WP_001270286.1 NZ_CP062758:2790737-2791153 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT176252 NZ_CP082774:479836-480132 [Escherichia coli O16:H6]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 6HPB