Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 358972..359603 Replicon chromosome
Accession NZ_CP062758
Organism Escherichia coli O157:H7 strain Z892

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag ILQ38_RS01635 Protein ID WP_001259386.1
Coordinates 358972..359247 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ILQ38_RS01640 Protein ID WP_000593555.1
Coordinates 359244..359603 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ38_RS01620 353976..355043 + 1068 WP_000361458.1 HlyD family secretion protein -
ILQ38_RS01625 355040..357775 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
ILQ38_RS01630 357775..358899 + 1125 WP_001301659.1 ABC transporter permease -
ILQ38_RS01635 358972..359247 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
ILQ38_RS01640 359244..359603 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ILQ38_RS01645 359653..360513 - 861 WP_001131788.1 class II aldolase -
ILQ38_RS01650 360545..360814 - 270 WP_000933060.1 HPr family phosphocarrier protein -
ILQ38_RS01655 360804..362312 - 1509 WP_206396595.1 carbohydrate kinase -
ILQ38_RS01660 362305..363663 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ILQ38_RS01665 363740..364021 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ILQ38_RS01670 364018..364491 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 350116..359603 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T176235 WP_001259386.1 NZ_CP062758:358972-359247 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T176235 NZ_CP082773:c1072723-1072346 [Escherichia coli O99:H6]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGATCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT176235 WP_000593555.1 NZ_CP062758:359244-359603 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT176235 NZ_CP082773:c1073144-1072770 [Escherichia coli O99:H6]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References