Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4201857..4202475 Replicon chromosome
Accession NZ_CP062752
Organism Escherichia coli O157:H7 strain Z910

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ILQ40_RS21230 Protein ID WP_001291435.1
Coordinates 4202257..4202475 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ILQ40_RS21225 Protein ID WP_000344800.1
Coordinates 4201857..4202231 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ40_RS21215 4196946..4198139 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ILQ40_RS21220 4198162..4201311 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
ILQ40_RS21225 4201857..4202231 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ILQ40_RS21230 4202257..4202475 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ILQ40_RS21235 4202647..4203198 + 552 WP_000102553.1 maltose O-acetyltransferase -
ILQ40_RS21240 4203314..4203784 + 471 WP_000136192.1 YlaC family protein -
ILQ40_RS21245 4203948..4205498 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ILQ40_RS21250 4205540..4205893 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ILQ40_RS21260 4206272..4206583 + 312 WP_000409911.1 MGMT family protein -
ILQ40_RS21265 4206616..4207188 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T176188 WP_001291435.1 NZ_CP062752:4202257-4202475 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T176188 NZ_CP082770:134-400 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT176188 WP_000344800.1 NZ_CP062752:4201857-4202231 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT176188 NZ_CP082770:5522-150 [Klebsiella pneumoniae]

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References