Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52615..53140 Replicon plasmid pZ1486-1
Accession NZ_CP062750
Organism Escherichia coli O157:H7 strain Z1486

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ41_RS27565 Protein ID WP_001159868.1
Coordinates 52835..53140 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ41_RS27560 Protein ID WP_000813634.1
Coordinates 52615..52833 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ41_RS27530 47898..48128 + 231 WP_010891288.1 hypothetical protein -
ILQ41_RS27535 48249..48989 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ41_RS27540 49274..50251 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ41_RS27545 50659..50859 - 201 WP_000708307.1 hypothetical protein -
ILQ41_RS27550 50856..51476 - 621 WP_001248529.1 hypothetical protein -
ILQ41_RS27555 51473..52156 - 684 WP_010891291.1 DNA-binding protein -
ILQ41_RS27560 52615..52833 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ41_RS27565 52835..53140 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ41_RS27570 53141..53947 + 807 WP_000016989.1 site-specific integrase -
ILQ41_RS27580 55845..56183 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
ILQ41_RS27585 56771..57937 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..94070 94070


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176161 WP_001159868.1 NZ_CP062750:52835-53140 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176161 NZ_CP082759:c4919295-4919011 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176161 WP_000813634.1 NZ_CP062750:52615-52833 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176161 NZ_CP082759:c4919527-4919285 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References