Detailed information of TA system TA176125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 51302..51827
• Replicon: plasmid pZ1504-1
• Accession: NZ_CP062747
• Organism: Escherichia coli O157:H7 strain Z1504

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: ILQ42_RS27490
• Protein ID: WP_001159868.1
• Coordinates: 51522..51827 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: ILQ42_RS27485
• Protein ID: WP_000813634.1
• Coordinates: 51302..51520 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ42_RS27455	46585..46815	+	231	WP_010891288.1	hypothetical protein	-
ILQ42_RS27460	46936..47676	+	741	WP_001066920.1	tyrosine-type recombinase/integrase	-
ILQ42_RS27465	47961..48938	-	978	WP_000361615.1	RepB family plasmid replication initiator protein	-
ILQ42_RS27470	49346..49546	-	201	WP_000708307.1	hypothetical protein	-
ILQ42_RS27475	49543..50163	-	621	WP_001248529.1	hypothetical protein	-
ILQ42_RS27480	50160..50843	-	684	WP_010891291.1	DNA-binding protein	-
ILQ42_RS27485	51302..51520	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
ILQ42_RS27490	51522..51827	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
ILQ42_RS27495	51828..52634	+	807	WP_000016989.1	site-specific integrase	-
ILQ42_RS27505	54532..54870	+	339	WP_071525396.1	RepB family plasmid replication initiator protein	-
ILQ42_RS27510	55458..56624	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB	1..92765	92765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T176125 WP_001159868.1 NZ_CP062747:51522-51827 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T176125 NZ_CP082360:c1376070-1375696 [Klebsiella sp. CTHL.F3a]
ATGCAAATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCAAAATCTGGCAAAAATTACTGGA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAACACATTG
ATGCGGGAATCTCACTGTGTGACGCTGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGCAGCCTTGATATGCTCCGAGACCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT176125 WP_000813634.1 NZ_CP062747:51302-51520 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT176125 NZ_CP082360:c1376468-1376109 [Klebsiella sp. CTHL.F3a]
ATGCAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACGTCACGCCATGTTT
TGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCACCACCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGTTACTGATGAAGCAGATGGAGCTCATGCTGACCAGCAGCGAACTCATG
CCCCGCATTCAGCGCTGCATCACGCTCCATGCAAAAGGGCTGATATGTAAGGCCGATACGCTCGGTTCCTGTGGCTATTT
GTATATCGTAATTTATCCGGCGTCTGCAACAACCGCATAA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2