Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5044419..5044916 Replicon chromosome
Accession NZ_CP062746
Organism Escherichia coli O157:H7 strain Z1504

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ILQ42_RS25100 Protein ID WP_001173343.1
Coordinates 5044419..5044592 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ILQ42_RS25105 Protein ID WP_000398619.1
Coordinates 5044620..5044916 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ42_RS25090 5041089..5042546 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
ILQ42_RS25095 5042783..5044300 + 1518 WP_001295074.1 lysine--tRNA ligase -
ILQ42_RS25100 5044419..5044592 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ILQ42_RS25105 5044620..5044916 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ILQ42_RS25110 5045143..5045415 - 273 WP_000405647.1 N-acetyltransferase -
ILQ42_RS25115 5045427..5045657 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ILQ42_RS25120 5045838..5047469 + 1632 WP_001216477.1 sensor histidine kinase -
ILQ42_RS25125 5047466..5048185 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176123 WP_001173343.1 NZ_CP062746:c5044592-5044419 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176123 NZ_CP082360:892557-892967 [Klebsiella sp. CTHL.F3a]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGCGCGCAGTGGTTCTCACTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTATTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATATTGCTGTCGCTGGTAGTCTTCGACTGCG
TACGCAGCCAGCGGCGGATCCACGCCCGTCAGGGTGAAATAAAGCTGCTGATCGACTCGCGTCTGCGCTGGCAAAAGGCA
GAGTGGGATATCATCGGCACGCCGTGGGTAATCAACAGCGGCATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGTAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGCGACCTGCGTCGGCTAGTGTTACAGAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176123 WP_000398619.1 NZ_CP062746:c5044916-5044620 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176123 NZ_CP082360:892310-892576 [Klebsiella sp. CTHL.F3a]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGTCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTAAGCGATGGCGATAAGGCGTTATTCATCCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCTGAACTGCAGCGGATGGTAAAATTAATTCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References