Detailed information of TA system TA176122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4947428..4948023
• Replicon: chromosome
• Accession: NZ_CP062746
• Organism: Escherichia coli O157:H7 strain Z1504

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: ILQ42_RS24600
• Protein ID: WP_000239579.1
• Coordinates: 4947428..4947778 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: ILQ42_RS24605
• Protein ID: WP_001223210.1
• Coordinates: 4947772..4948023 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ42_RS24580	4942874..4943896	-	1023	WP_001301928.1	ABC transporter permease	-
ILQ42_RS24585	4943910..4945412	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
ILQ42_RS24590	4945552..4946508	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ILQ42_RS24595	4946818..4947348	+	531	WP_000055075.1	inorganic diphosphatase	-
ILQ42_RS24600	4947428..4947778	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
ILQ42_RS24605	4947772..4948023	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
ILQ42_RS24610	4948235..4948576	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
ILQ42_RS24615	4948579..4952358	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T176122 WP_000239579.1 NZ_CP062746:c4947778-4947428 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T176122 NZ_CP082359:c1651806-1651543 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTCAAGCGGAGCGCTAATAAAGATCTCAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACCCCTTGGCACCCAACCAAGGCTTTGAAAAATTAGTACTGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGCACCGACTCGTCTATAAGGTTGATCAAGACACGCAAACTGTGATCATCTAT
TCTGCGTGGTCACATGACGAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT176122 WP_001223210.1 NZ_CP062746:c4948023-4947772 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT176122 NZ_CP082359:c1652057-1651803 [Limosilactobacillus fermentum]
ATGGACCAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
GGTTGTGATTAAGCCGACCAAGGCGGATGAAAAGAGCGTTGTCGTCATTGGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTTTGGTTAATCACGGTGTTGACAAGCAAATTAAAGAACGGGAAAACGAGCCGCTCGACGACTTTGATGATGTT
TGGGGTCAACTATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8