Detailed information of TA system TA176114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3578249..3578727
• Replicon: chromosome
• Accession: NZ_CP062746
• Organism: Escherichia coli O157:H7 strain Z1504

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: ILQ42_RS18400
• Protein ID: WP_001303876.1
• Coordinates: 3578440..3578727 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: ILQ42_RS18395
• Protein ID: WP_000536233.1
• Coordinates: 3578249..3578440 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ42_RS18360	3573653..3574423	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ42_RS18365	3574445..3575191	-	747	WP_000788751.1	ATP-binding protein	-
ILQ42_RS18370	3575198..3576289	-	1092	WP_001205823.1	hypothetical protein	-
ILQ42_RS18375	3576368..3576823	+	456	WP_000273724.1	hypothetical protein	-
ILQ42_RS18380	3577030..3577455	-	426	WP_000693855.1	Rha family transcriptional regulator	-
ILQ42_RS18385	3577439..3577711	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
ILQ42_RS18390	3577820..3578221	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
ILQ42_RS18395	3578249..3578440	+	192	WP_000536233.1	hypothetical protein	Antitoxin
ILQ42_RS18400	3578440..3578727	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ILQ42_RS18405	3579005..3579160	+	156	WP_000379575.1	DUF1391 family protein	-
ILQ42_RS18410	3579302..3579691	+	390	WP_000394511.1	hypothetical protein	-
ILQ42_RS18415	3579878..3580063	-	186	WP_001133046.1	hypothetical protein	-
ILQ42_RS18420	3580637..3580825	+	189	WP_000413705.1	cell division inhibition protein DicB	-
ILQ42_RS18425	3580822..3581013	+	192	WP_001098307.1	DUF1482 family protein	-
ILQ42_RS18430	3581107..3583578	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T176114 WP_001303876.1 NZ_CP062746:3578440-3578727 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T176114 NZ_CP082357:3499895-3500437 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT176114 WP_000536233.1 NZ_CP062746:3578249-3578440 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT176114 NZ_CP082357:3499600-3499911 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA