Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2856118..2856756 Replicon chromosome
Accession NZ_CP062746
Organism Escherichia coli O157:H7 strain Z1504

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ILQ42_RS14505 Protein ID WP_000813794.1
Coordinates 2856118..2856294 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ILQ42_RS14510 Protein ID WP_001270286.1
Coordinates 2856340..2856756 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ42_RS14485 2851740..2852951 - 1212 WP_071525382.1 BenE family transporter YdcO -
ILQ42_RS14490 2853004..2853540 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
ILQ42_RS14495 2853613..2855574 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ILQ42_RS14500 2855666..2855896 - 231 WP_000494244.1 YncJ family protein -
ILQ42_RS14505 2856118..2856294 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ILQ42_RS14510 2856340..2856756 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ILQ42_RS14515 2856835..2858241 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
ILQ42_RS14520 2858486..2859631 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
ILQ42_RS14525 2859649..2860662 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
ILQ42_RS14530 2860663..2861604 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T176101 WP_000813794.1 NZ_CP062746:2856118-2856294 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T176101 NZ_CP082355:c4569604-4569293 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT176101 WP_001270286.1 NZ_CP062746:2856340-2856756 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT176101 NZ_CP082355:c4569292-4569002 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References