Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51291..51816 Replicon plasmid pZ1626-1
Accession NZ_CP062743
Organism Escherichia coli O157:H7 strain Z1626

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ44_RS27550 Protein ID WP_001159868.1
Coordinates 51511..51816 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ44_RS27545 Protein ID WP_000813634.1
Coordinates 51291..51509 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ44_RS27515 46575..46805 + 231 WP_010891288.1 hypothetical protein -
ILQ44_RS27520 46926..47666 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ44_RS27525 47951..48927 - 977 Protein_45 replication initiation protein -
ILQ44_RS27530 49335..49535 - 201 WP_000708307.1 hypothetical protein -
ILQ44_RS27535 49532..50152 - 621 WP_001248529.1 hypothetical protein -
ILQ44_RS27540 50149..50832 - 684 WP_010891291.1 DNA-binding protein -
ILQ44_RS27545 51291..51509 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ44_RS27550 51511..51816 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ44_RS27555 51817..52623 + 807 WP_000016989.1 site-specific integrase -
ILQ44_RS27565 54521..54859 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
ILQ44_RS27570 55447..56613 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / espP / espP / stcE / exeE / exeF / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92746 92746


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176049 WP_001159868.1 NZ_CP062743:51511-51816 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176049 NZ_CP082347:266020-266364 [Pantoea dispersa]
ATGCAAGCGACGTTTATTGAGCTTTCCAGCTTTCAGAAATACCGGGCAGATTACCTGAGCGACGATCAATTCAGGCTGTT
TCAGAATATGTTGCTGGCCGACCCCGAAAAGGGCGACGTCATTCCCGATACGGGCGGGCTTCGTAAAGTTCGTTTCAGGG
ATGAACGCCGGAACAAGGGATCGCGGGGCGGGATTAGAGTTATCTATTACTGGACGAATGAAAAAGGCCAGTTCATCCTG
TTCAGCATATATGATAAAGATCAACGCGACGATCTGACCAAACAACAGCGCGATGCCCTGGCGGAGGCGCTGAACGTGAT
CAAAAAGGGGCTGAGACATGACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176049 WP_000813634.1 NZ_CP062743:51291-51509 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176049 NZ_CP082347:266357-266671 [Pantoea dispersa]
ATGACTGAACGCGATATTTTCAGTGAGCTGATGGCGGGTATGCAGGAGCTGAAAGACCACCAGGACGGAAAAATCACGCT
CAAGACCTATAAGGTCAGTAAGCGCGCGCCTGTCACCATTGCGCCGCAGGAGCTGCGCGACGTGCGCGAAAAGCTTAACC
TGTCACAGGCCGTTTTTGCGCACTATCTTCACACGGGCGAAACCACCTATCAGAACTGGGAGCAGGGCAGGGCAAAACCT
AACGCTCAGGCCGTACTGCTGATTCGCATGGTGCAGCAAAATCCTGAAACACTGCAGACTCTCGCGCAATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References