Detailed information of TA system TA176046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4959318..4959913
• Replicon: chromosome
• Accession: NZ_CP062742
• Organism: Escherichia coli O157:H7 strain Z1626

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: ILQ44_RS24660
• Protein ID: WP_000239579.1
• Coordinates: 4959318..4959668 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: ILQ44_RS24665
• Protein ID: WP_001223210.1
• Coordinates: 4959662..4959913 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ44_RS24640	4954764..4955786	-	1023	WP_001301928.1	ABC transporter permease	-
ILQ44_RS24645	4955800..4957302	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
ILQ44_RS24650	4957442..4958398	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ILQ44_RS24655	4958708..4959238	+	531	WP_000055075.1	inorganic diphosphatase	-
ILQ44_RS24660	4959318..4959668	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
ILQ44_RS24665	4959662..4959913	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
ILQ44_RS24670	4960125..4960466	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
ILQ44_RS24675	4960469..4964248	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T176046 WP_000239579.1 NZ_CP062742:c4959668-4959318 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T176046 NZ_CP082346:3589489-3589953 [Pantoea dispersa]
GTGATTTTTTATCGGCTGACGAAGAGCCAGTATGCGGCTGAAGCCTGGTCCGGTTATGGGGCAAAACAGTATGGTGGACG
CTGGAATCATAAAGGCTATCCCACCGTTTATGTTGCCACGTCGGTGTCACTGGCGGCACTGGAAGTGCTGGTTCATATCG
GAAGAGAGTCGGTGCTGGATGAGTACACCTTACTCAGTATCGACATCCCGGATGCAGAAGTTGCCTATCTTGCTGAAGCA
TTTTTGCCGCCGGACTGGCGTCATGATCCAGCGCCACTCTCGACAATGGATCTCGGCACCGGCTGGCTGCAATCCGCTGA
AGGCGCCGCGCTGATGCTACCTTCCACGATTATCCCGATGGAAAACAACGCCATTCTGAACCCTCTCCATCCTGCCTTTA
GTGACTATCTGCCGGGCGTGAAAGCGTTACCGTTTATGTTTGATCGCCGGCTTATCAGGACATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT176046 WP_001223210.1 NZ_CP062742:c4959913-4959662 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT176046 NZ_CP082346:3589049-3589492 [Pantoea dispersa]
ATGCGAGCTTATATTCCTCAGCCTGGGGCCAATGACAACGCGCTGTGGCGGGTTGCAGGCCTGCCTCCACGTGGTATTGA
GTTAACGCGTATGCTGGAAGCCGGTTTGCCGGTGGCCGTCATTGATGATATCCGTCACTGGTCGGCCATGACGCGCGCCG
AAATCATGAAGGTCACAGGGATTAATGAACGCAACATTGCCCGTCGTAAAAGCACGGGTAAAAGTCTGTCACCCGACGAG
AGTGAACGTGTCGCCCGCTTCGTCCGCGTGATGGATGCCGCCGTTCAGCTTTTTGCTGGTAATAAAGAAGAGGCCACCCA
GTGGCTGAGCCGACCGGTGAAAGGGTTAGGCAATGTTGCGCCCATCACCTTACTGACGACGGAGAGCGGTGCGCTGGATG
TGCTCGATTTGATCGGCCGTCTGGAGCACGGTGTTTTCTCGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8