Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4213667..4214285 Replicon chromosome
Accession NZ_CP062742
Organism Escherichia coli O157:H7 strain Z1626

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ILQ44_RS21290 Protein ID WP_001291435.1
Coordinates 4214067..4214285 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ILQ44_RS21285 Protein ID WP_000344800.1
Coordinates 4213667..4214041 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ44_RS21275 4208756..4209949 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ILQ44_RS21280 4209972..4213121 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
ILQ44_RS21285 4213667..4214041 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ILQ44_RS21290 4214067..4214285 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ILQ44_RS21295 4214457..4215008 + 552 WP_000102553.1 maltose O-acetyltransferase -
ILQ44_RS21300 4215124..4215594 + 471 WP_000136192.1 YlaC family protein -
ILQ44_RS21305 4215758..4217308 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ILQ44_RS21310 4217350..4217703 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ILQ44_RS21320 4218082..4218393 + 312 WP_000409911.1 MGMT family protein -
ILQ44_RS21325 4218426..4218998 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T176040 WP_001291435.1 NZ_CP062742:4214067-4214285 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T176040 NZ_CP082342:c586842-586465 [Pantoea dispersa]
ATGAATCATGTTTCAGCCGATGAAGTCATTGCGCTTCACGATTATGTGTTGAGACGTTATGCCGGTGTTACGGGAATGCA
GGATCCGGGCCGTGCGCAAGCCATCGTTACCCGAGTCATTAATCGGCAGTTTTACGAAGGTGTGCAGGATGTTTACGAGC
TCGCTGCAACCTATTGGGTTGCTATTTCTCGTGGTCACATTTTTGCTGACGCTAATAAACGGACTTCTCTCAACGTCACC
ATGCTGTTTCTGAAACGCAACGGGGTAAAGGTGCATGATCGGCCAGAGTTAGTTGAGCTGACCGTTATGGCTGCCACCGG
TGAAGCGGGCATTGACGATCTTGCCCGCGCATTACGCTCATTCTTTAGCGCTCATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT176040 WP_000344800.1 NZ_CP062742:4213667-4214041 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT176040 NZ_CP082342:c587060-586839 [Pantoea dispersa]
ATGAATAAATTCACCTATACCAGCGCGCGTGAGAATTTTTCTCAGGTGCTTGACCAGGCCATTGCGGGCGAAGCTGTGGA
AATTACACGTCGCAGCGGCGATTCTGCCGTCGTCATTAGCAAAGCGCATTTTGATGCCTATCGTAAAGCTGCGCTTGATG
CAGAGTTTGCCGAACTGATCGGGGAGTTTGATGCCTCAAATCAGGCGCTGGCCGATAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References