Detailed information of TA system TA176034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2850995..2851633
• Replicon: chromosome
• Accession: NZ_CP062742
• Organism: Escherichia coli O157:H7 strain Z1626

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ILQ44_RS14490
• Protein ID: WP_000813794.1
• Coordinates: 2851457..2851633 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ILQ44_RS14485
• Protein ID: WP_001270286.1
• Coordinates: 2850995..2851411 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ44_RS14465	2846147..2847088	-	942	WP_001251315.1	ABC transporter permease	-
ILQ44_RS14470	2847089..2848102	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
ILQ44_RS14475	2848120..2849265	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
ILQ44_RS14480	2849510..2850916	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
ILQ44_RS14485	2850995..2851411	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ILQ44_RS14490	2851457..2851633	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ILQ44_RS14495	2851855..2852085	+	231	WP_000494244.1	YncJ family protein	-
ILQ44_RS14500	2852177..2854138	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ILQ44_RS14505	2854211..2854747	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
ILQ44_RS14510	2854800..2856011	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T176034 WP_000813794.1 NZ_CP062742:c2851633-2851457 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T176034 NZ_CP082341:c4037096-4036797 [Pantoea dispersa]
ATGCAAACGGAACCGGAGTTTGAGTGGGATACCACCAAGGCGGAAACAAATTTCCGCAAGCATGGTATCCGCTTTGAAGT
TGCAGCCCGCGTGTTTGAGGATCCTTTCCACCTGTCGGTGCAGGATCGTTATGAAAATGGGGAATATCGCTGGCAAACCA
TTGGTAATGTTATGGAGCATGTGGTCATTCTGGTGGCCCATACGGTGCGCTTCGAAACAGATGTTGAGCGAATAAGGATT
ATCAGCGCACGCCATGCAACGCGTATTGAGAGGAGGCGCTATGAGCAAAGTCAGGTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT176034 WP_001270286.1 NZ_CP062742:c2851411-2850995 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT176034 NZ_CP082341:c4036816-4036514 [Pantoea dispersa]
ATGAGCAAAGTCAGGTTTAAACGCAGTGAATTACCGCCGCTCACCGATAAGCAACAGGCGGAGCTGGATGCTTTGAGCCG
GAAACAGGACAGCGACATCCAGTGTGATGATATTGCGCCGTTAGACGACAGTTTCTGGCAGGCTGCCGAACGGGGGCGTT
TTTACCGCCCGGTAAAAACCCAGGCTTCGATCAGAATCGATGCGGATATTCTTGAGTGGTTGAAGGCGCCTGGCAAAGGT
TACCAGACGCGCCTCAACGCGATCCTGCGAGCCGCGATGCTAAGAGAACTGCAGGGTAAATAG

Structures

Toxin

• PDB: 6HPB