Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 53752..54277 Replicon plasmid pZ1766-1
Accession NZ_CP062737
Organism Escherichia coli O157:H7 strain Z1766

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ46_RS27580 Protein ID WP_001159868.1
Coordinates 53972..54277 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ46_RS27575 Protein ID WP_000813634.1
Coordinates 53752..53970 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ46_RS27545 49035..49265 + 231 WP_010891288.1 hypothetical protein -
ILQ46_RS27550 49386..50126 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ46_RS27555 50411..51388 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ46_RS27560 51796..51996 - 201 WP_000708307.1 hypothetical protein -
ILQ46_RS27565 51993..52613 - 621 WP_001248529.1 hypothetical protein -
ILQ46_RS27570 52610..53293 - 684 WP_010891291.1 DNA-binding protein -
ILQ46_RS27575 53752..53970 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ46_RS27580 53972..54277 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ46_RS27585 54278..55084 + 807 WP_000016989.1 site-specific integrase -
ILQ46_RS27595 56982..57320 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
ILQ46_RS27600 57908..59074 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..95229 95229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T175975 WP_001159868.1 NZ_CP062737:53972-54277 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T175975 NZ_CP082312:c11506-11204 [Vibrio alginolyticus]
ATGGCACAATATAAACTTTCTCCTGCCGCACAAACTGACCTAGTCGATATTCGTCGTTACACTCTTGAAAATTGGGGTAG
CGCACAGTGGACTAATTACTTCGGGGAACTAAAGCGTTCGATGGCACTATTAGCTAACAACGAGTTGATTGGTATCGATA
TGCCCGAACTGGGAACAGGCTATTGTCGCTTTCCATTAAAACATCATGTAATTTACTACATAAAAAAATCTGACCATATT
GTGGTTGCTGCGGTTCTTGGCCGAAATATGTCTCCTGCTAAACATTTCCAGCAACAGATATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT175975 WP_000813634.1 NZ_CP062737:53752-53970 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT175975 NZ_CP082312:c11766-11506 [Vibrio alginolyticus]
ATGCAATCATTAACCGCAAATACCGCGAAAACTAAATTTGGTGATTTACTGATGAAAGTACAGCGAGAACCAGTACAAAT
TAGTAAAAATGGCTCACCGGTGGCGGTCGTCATGTCATGTGAAGAATACGAACAACTTGAAGCTTTGAAAATGGCGATGG
TGAAATCACGTTTTGAACAAGCTGAGCGAGATATCGCAAATGGCGATTTGGTCGATGGTGAAACATTTATGAACGAATTG
GACCAGGGCAAGTTTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References