Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5053835..5054332 Replicon chromosome
Accession NZ_CP062736
Organism Escherichia coli O157:H7 strain Z1766

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ILQ46_RS25180 Protein ID WP_001173343.1
Coordinates 5053835..5054008 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ILQ46_RS25185 Protein ID WP_000398619.1
Coordinates 5054036..5054332 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ46_RS25170 5050505..5051962 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
ILQ46_RS25175 5052199..5053716 + 1518 WP_001295074.1 lysine--tRNA ligase -
ILQ46_RS25180 5053835..5054008 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ILQ46_RS25185 5054036..5054332 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ILQ46_RS25190 5054559..5054831 - 273 WP_000405647.1 N-acetyltransferase -
ILQ46_RS25195 5054843..5055073 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ILQ46_RS25200 5055254..5056885 + 1632 WP_001216477.1 sensor histidine kinase -
ILQ46_RS25205 5056882..5057601 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T175973 WP_001173343.1 NZ_CP062736:c5054008-5053835 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T175973 NZ_CP082309:1416-1733 [Vibrio alginolyticus]
ATGCACGTAATTAAACGAGAGCCTTTTGAAACGGCAAAGCGACTATATCCGCAATGCGCCGAGGCACTAGACTCAACTTT
CAAAACGCTAAAAGCAACAAAAGCAAAAACACCAGAACAACTAAAACAACTTTTTCCATCATTGGATAACTTTAAATACG
TAGACAAGTGGTATGTCATCGACATTGGCGGAGGTTCATTAAGATTAATCGCCTTTATCGAATTTACAGGTGGGAAATGC
TTCGTTAAACACATCGTTACCCACGCAGAATACGACAAGATAACAGACCAACACAGAACAAAGGGGAAAAGGGGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT175973 WP_000398619.1 NZ_CP062736:c5054332-5054036 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT175973 NZ_CP082309:1736-2149 [Vibrio alginolyticus]
ATGAACATTCAAGCTCTAAAAACCCTATCAAATGAGATTCACACAGCGATGCCTTGGTTACAAGGCATCCGTGACCATGA
GCAATACCTAGAGCTTATGGAGCTCATGGATACGCTAGTGGAAGACTACGAGGAAAACCATGTCTTGATTGACTTGCTAT
TCCCTGTACTTGAACGCTACGAGGAAGAATCAGAACACTTCCAAGAGTTTAACGCGCACATTGATAGCCTAGATACTGGT
ATCGCTACTCTTCGCGTTCTCATCGACCAATACCGCCTAACTCTAAGTGATTTACCTGAGATTGGTGGCAAGTCGCTAGT
ATCGCTAATCCTAAGCGGTAAACGCCAACTAACCGTTGGACACATTAAAGCGCTATCGGAACGTTTCGGCGTGCCAAAGC
ATATGTTTATTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References