Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2466622..2467260 Replicon chromosome
Accession NZ_CP062733
Organism Escherichia coli O157:H7 strain Z1767

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ILQ47_RS12380 Protein ID WP_000813794.1
Coordinates 2466622..2466798 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ILQ47_RS12385 Protein ID WP_001270286.1
Coordinates 2466844..2467260 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ47_RS12360 2462244..2463455 - 1212 WP_071525382.1 BenE family transporter YdcO -
ILQ47_RS12365 2463508..2464044 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
ILQ47_RS12370 2464117..2466078 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ILQ47_RS12375 2466170..2466400 - 231 WP_000494244.1 YncJ family protein -
ILQ47_RS12380 2466622..2466798 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ILQ47_RS12385 2466844..2467260 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ILQ47_RS12390 2467339..2468745 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
ILQ47_RS12395 2468990..2470135 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
ILQ47_RS12400 2470153..2471166 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
ILQ47_RS12405 2471167..2472108 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T175914 WP_000813794.1 NZ_CP062733:2466622-2466798 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T175914 NZ_CP082267:1094380-1094646 [Enterococcus lactis]
ATGACCTACCGTTTAGAATTCACGCCTGAAATTCAGAAACAACTTCGTAAAATGGACAAGCATCAAGCGACGCTGATCGT
TCGATGGCTGTATCAACATATTGATGGTATTGACGATCCGAGAAAATTCGGAAAAGGATTGACCGCTAATCGTTCAGGAC
AATGGCGTTATCGAATTGGCAAATATCGTGTGTTGGTAGAGATAGAAGATAATCAAATGGTTGTTATCGCTATTCAAGTG
GGACACCGAAAAAATGTCTATGATTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT175914 WP_001270286.1 NZ_CP062733:2466844-2467260 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT175914 NZ_CP082267:1094153-1094383 [Enterococcus lactis]
ATGGCTATGATTACGGTTCGTGTGAGTGATGCCGAAAAAGAATGGCTTTCTTATATGGCGGAATTTTATGGGATTACTTT
ATCTGAGCTTCTAAAAACCTATTCGATGGAACAACTAGAAGATGAATACGATCGTCAAACGGCTGAAATTGCCCATAAAC
ATTGGGTAGAAAGTGGAAAAACAACAGTATCCATGGAAGAAATCCTTCATGAATTTGGAGGGTTGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References