Detailed information of TA system TA175905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 431606..433934
• Replicon: chromosome
• Accession: NZ_CP062733
• Organism: Escherichia coli O157:H7 strain Z1767

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: ILQ47_RS02035
• Protein ID: WP_000532146.1
• Coordinates: 431606..432580 (+)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: ILQ47_RS02040
• Protein ID: WP_001303704.1
• Coordinates: 432639..433934 (+)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ47_RS02010	427463..428458	+	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-
ILQ47_RS02015	428597..429124	+	528	WP_000108330.1	gluconokinase	-
ILQ47_RS02020	429128..430468	+	1341	WP_000210111.1	gluconate transporter	-
ILQ47_RS02025	430608..431216	+	609	WP_000410808.1	ATP-dependent DNA helicase	-
ILQ47_RS02030	431201..431602	+	402	WP_000502498.1	ATP-dependent DNA helicase	-
ILQ47_RS02035	431606..432580	+	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
ILQ47_RS02040	432639..433934	+	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
ILQ47_RS02045	433980..434573	-	594	WP_001002544.1	NAAT family transporter YhgN	-
ILQ47_RS02050	434765..435868	+	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
ILQ47_RS02055	436141..438327	+	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T175905 WP_000532146.1 NZ_CP062733:431606-432580 [Escherichia coli O157:H7]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T175905 NZ_CP082238:c2688600-2688316 [Acidithiobacillus ferrooxidans]
GTGGTTTGTGAATTACGGTATGGCGCAGCAAAGAAAAATTCTCCCCGACTCACAGAACAGGTGAACGCCGTTATCGCGGC
CATGGATGTGCTCCCCTATGACACGCCGGTCGATCAGGCTTATGCGGAAATCCGTCAACACCTTGCCGCCTACGGCCAGC
CCATTGGTCCGAATGACTTGCTTATTGCCTCCCATGCACTGGCAGTCAAGGCCATATTGGTGAGCGCCAATGTGGGCGAG
TTTTCCCGAGTGCCCGGGCTCATAGTGCACAATTGGCTGCAGTGA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT175905 WP_001303704.1 NZ_CP062733:432639-433934 [Escherichia coli O157:H7]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT175905 NZ_CP082238:c2688960-2688721 [Acidithiobacillus ferrooxidans]
ATGCATACGGAACGCCATGTACGTTTTTTTCGGAATGGCCGCAATCAAGCGGTTCGCATTCCCCGTGAGTTCGAGATGGA
CGCGCAAGAGGCCATCATGCGGCGTGAGGATGATCGCTTGATTATCGAGCCCGTAAAACGCAAAGGGCTTCTGGCCACTC
TGGCGAGTCTTTCTGCCTTGGATGAGGAGTTCCCGGATACGGATCGCGATCTTCTCCCGCTGGATGACATCGAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8