Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5053834..5054331 Replicon chromosome
Accession NZ_CP062731
Organism Escherichia coli O157:H7 strain Z1768

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ILQ48_RS25170 Protein ID WP_001173343.1
Coordinates 5053834..5054007 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ILQ48_RS25175 Protein ID WP_000398619.1
Coordinates 5054035..5054331 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ48_RS25160 5050504..5051961 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
ILQ48_RS25165 5052198..5053715 + 1518 WP_001295074.1 lysine--tRNA ligase -
ILQ48_RS25170 5053834..5054007 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ILQ48_RS25175 5054035..5054331 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ILQ48_RS25180 5054558..5054830 - 273 WP_000405647.1 N-acetyltransferase -
ILQ48_RS25185 5054842..5055072 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ILQ48_RS25190 5055253..5056884 + 1632 WP_001216477.1 sensor histidine kinase -
ILQ48_RS25195 5056881..5057600 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T175899 WP_001173343.1 NZ_CP062731:c5054007-5053834 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T175899 NZ_CP082238:c1381418-1380924 [Acidithiobacillus ferrooxidans]
ATGAGTTTGAAGCTATCCGCGCCGCAGCCACTTGCGGCCGTGCACCTGTTGGGCGACTTCGAGAGCGGTGAGCCTGCACT
GGATGAGTGGTTAAAACGCCGGGCGATGAACAACCAGCTCACCGGGGCCAGTCGAACCTTTGTGGTCGTTGATGAAGAAA
ATCGGGTGCGCGGCTTCTACGCCATGGCGGCTGGCGCAGTCGCACATCAAGTGGCGACTAGCTCGGTTCGTCGCAATATG
CCCGATCCGGTTCCCGTCATGGTGCTCGGTAGGTTGGCGGTGGATAGTCGAGCGCAAGGCATCAAGCTGGGGGCGGCCAT
GCTGCAGGACGCGGTGAAACGTGCCATCACCGTATCGCAGAACACGGGGGTTCGTGCACTGCTGGTTCATGCGCTCCACG
AACGCGCCAAACAGTTCTATGAGCACTACGGCTTTCAGGAGTCACCACAACATCCGATGACGCTGATGTTGCGCCTGAAT
AGCATCAAGGCTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT175899 WP_000398619.1 NZ_CP062731:c5054331-5054035 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT175899 NZ_CP082238:c1381687-1381415 [Acidithiobacillus ferrooxidans]
ATGCGTGCCGCTGCCATCAATTTACGAGCCTTGCCGGAGCAGCGTGATCTCATTGATCACGCCGCCAACCTGTTAGGCAA
AAACCGTTCGGACTTCATGCTGGAAGCCGCCTGCGAGCGTGCGCAATCGGTACTGCTCGATCAGGTGTTTTTTGGGCTGG
ATGCGGAAAAGTTCCGGCAGTTCACCGCCATGCTCGACGCACCGCCTCGTGGCAACGAAGGTCTTGAGCGTCTGATGGCA
GTCAAGGCGCCATGGGAAAGCAATCAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References