Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51301..51826 Replicon plasmid pZ1811-1
Accession NZ_CP062730
Organism Escherichia coli O157:H7 strain Z1811

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ50_RS27550 Protein ID WP_001159868.1
Coordinates 51521..51826 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ50_RS27545 Protein ID WP_000813634.1
Coordinates 51301..51519 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ50_RS27515 46584..46814 + 231 WP_010891288.1 hypothetical protein -
ILQ50_RS27520 46935..47675 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ50_RS27525 47960..48937 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ50_RS27530 49345..49545 - 201 WP_000708307.1 hypothetical protein -
ILQ50_RS27535 49542..50162 - 621 WP_001248529.1 hypothetical protein -
ILQ50_RS27540 50159..50842 - 684 WP_010891291.1 DNA-binding protein -
ILQ50_RS27545 51301..51519 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ50_RS27550 51521..51826 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ50_RS27555 51827..52633 + 807 WP_000016989.1 site-specific integrase -
ILQ50_RS27565 54531..54869 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
ILQ50_RS27570 55457..56623 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92757 92757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T175865 WP_001159868.1 NZ_CP062730:51521-51826 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T175865 NZ_CP082218:c4217891-4217739 [Xanthomonas cucurbitae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGCGCCGGCAACAAGGTCGAGAAGACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT175865 WP_000813634.1 NZ_CP062730:51301-51519 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT175865 NZ_CP082218:c4217661-4217527 [Xanthomonas cucurbitae]
ATGAAGCGTGCAATCGTGCTGCTGGTGCTGTCAGTGTTGTCGGTCGGGATGCTGTCAGGTTGCAACACCGTCGCAGGTGC
CGGCAAAGACGTGCAGGGCGCCGGCGAGAAGGTCGAAGACGCCGCACGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References