Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3060917..3061388 Replicon chromosome
Accession NZ_CP062729
Organism Escherichia coli O157:H7 strain Z1811

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ILQ50_RS15535 Protein ID WP_001303511.1
Coordinates 3061110..3061388 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ILQ50_RS15530 Protein ID WP_001302048.1
Coordinates 3060917..3061108 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ50_RS15490 3056124..3056708 - 585 WP_000206793.1 DUF551 domain-containing protein -
ILQ50_RS15495 3056765..3057160 - 396 WP_001118159.1 DUF977 family protein -
ILQ50_RS15500 3057176..3057945 - 770 Protein_3031 DUF1627 domain-containing protein -
ILQ50_RS15505 3057971..3058711 - 741 WP_000788938.1 ATP-binding protein -
ILQ50_RS15510 3058718..3059680 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
ILQ50_RS15515 3059703..3060128 - 426 WP_000693943.1 Rha family transcriptional regulator -
ILQ50_RS15520 3060125..3060427 - 303 WP_000172738.1 transcriptional regulator -
ILQ50_RS15525 3060525..3060896 + 372 WP_001169686.1 hypothetical protein -
ILQ50_RS15530 3060917..3061108 + 192 WP_001302048.1 hypothetical protein Antitoxin
ILQ50_RS15535 3061110..3061388 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ILQ50_RS15540 3061741..3062040 + 300 WP_001240334.1 hypothetical protein -
ILQ50_RS15545 3062112..3062330 + 219 WP_001171930.1 hypothetical protein -
ILQ50_RS15550 3062899..3063087 + 189 WP_000449192.1 cell division inhibition protein DicB -
ILQ50_RS15555 3063084..3063275 + 192 WP_001090200.1 DUF1482 family protein -
ILQ50_RS15560 3063368..3065839 + 2472 WP_072612735.1 exonuclease -
ILQ50_RS15565 3065904..3066152 + 249 WP_000113189.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleC / nleG7' 2926140..3069044 142904


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T175850 WP_001303511.1 NZ_CP062729:3061110-3061388 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T175850 NZ_CP082215:c4280053-4279901 [Xanthomonas cucurbitae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGCGCCGGCAACAAGGTCGAGAAGACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT175850 WP_001302048.1 NZ_CP062729:3060917-3061108 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT175850 NZ_CP082215:c4279823-4279689 [Xanthomonas cucurbitae]
ATGAAGCGTGCAATCGTGCTGCTGGTGCTGTCAGTGTTGTCGGTCGGGATGCTGTCAGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCCGGCGAGAAGGTGGAAGACGCCGCACGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References