Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2802462..2803100 Replicon chromosome
Accession NZ_CP062729
Organism Escherichia coli O157:H7 strain Z1811

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ILQ50_RS14100 Protein ID WP_000813794.1
Coordinates 2802462..2802638 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ILQ50_RS14105 Protein ID WP_001270286.1
Coordinates 2802684..2803100 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ50_RS14080 2798084..2799295 - 1212 WP_071525382.1 BenE family transporter YdcO -
ILQ50_RS14085 2799348..2799884 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
ILQ50_RS14090 2799957..2801918 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ILQ50_RS14095 2802010..2802240 - 231 WP_000494244.1 YncJ family protein -
ILQ50_RS14100 2802462..2802638 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ILQ50_RS14105 2802684..2803100 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ILQ50_RS14110 2803179..2804585 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
ILQ50_RS14115 2804830..2805975 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
ILQ50_RS14120 2805993..2807006 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
ILQ50_RS14125 2807007..2807948 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T175847 WP_000813794.1 NZ_CP062729:2802462-2802638 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T175847 NZ_CP082215:1901440-1901646 [Xanthomonas cucurbitae]
ATGCAGGCCGCAGCCGAGCGGAAGCTGCAGTTGCTGGATTCGGCGCAGACGCTGGAGTTCTTGCGCAGTCCGCCAGCCAA
TCGGCTTGAGCTTCTTGCAGGTACGCGTGTAGGTCAGCACAGCATCCGCATCAACGACCAATGGCGTGTGTGCTTTGTCT
GGACCGGTGCCGGCCCTGGGCACGTAGAGATTGTTGACTATCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT175847 WP_001270286.1 NZ_CP062729:2802684-2803100 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT175847 NZ_CP082215:1901656-1901955 [Xanthomonas cucurbitae]
ATGCGACCCACCAACAAGCTGCGCGCGATCCATCCCGGCGAGATCCTGCGCGAGGACTTCATGCTGCCATTGCAGCTGAC
CATCAACGGCCTGGCGCGTGCGTTGGATGTGCCTGCCACGCGCGTCCACAGCATCGTGCACGGCGCGCGCGCGATCACTG
CCGATACCGCTGCGCGCCTTGCGCGTCACTTCGGTGGCGACGCGGCATCCTGGCTTGCGCTGCAGGCCACTTACGACCTG
AAGACCTTGCCCAACCGTAGTGAGATCGAGACCAACGTGCAGCCGCGTGTAGCGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References